Use the invitrogen dna ladder selection table below to help you choose the dna ladder you need invitrogen egel dna ladders are formulated specifically for maximum performance on invitrogen egel precast agarose gels and are provided in readytoload formats. These ladders are sometimes made from restriction enzyme digestion of plasmid dna with a known sequence. This ladder is stored at 20c for at least one year. Promega corpora on 2800 woods hollow road madison, wi 537115399 usa toll free in usa 8003569526 6082744330 fax 6082772516 5 tm367 1211 6.
Quickload purple dna ladders from neb are premixed, readytoload molecular weight markers. Generuler 1 kb dna ladder thermo fisher scientific. The wizard genomic dna purification kit is based on a fourstep process. The benchtop dna markers offer the convenience of storage at room temperature 2225c as well as the capability of direct loading onto agarose gels. The 1kb dna ladder has thirteen bluntended fragments with sizes ranging from 250bp to 10,000bp. The quickload purple 1 kb dna ladder is not intended for precise quantification of dna mass but can be used for approximating the mass of dna in comparably intense samples of similar size.
An update to dna ladder assay for apoptosis detection article pdf available in bioimpacts 51. Quickly determine the size of your dna, using evenlyspaced bands and easilyidentifiable reference bands. Forty bluntended dna fragments ranging from 100bp to 4,000bp in 100bp increments. After treatment of cells with apoptotic agent, 500. Generuler 100 bp dna ladder 250 5 x 50 g for 500 applications, 0. Once the researcher has amplified a dna fragment, he. Dna mass ladders are specifically created for quantitative estimation of dna mass in gels. In addition, dnapkcs may help recruit dna repair machinery to damaged telomeres. Here we introduce a rapid and improved method of dna ladder apoptosis assay for evaluating apoptosis in mammalian cells. How can i prepare working solution with 1 kb dna ladder.
Generuler 100 bp dna ladder thermo fisher scientific. Download pdf promega forensic products are manufactured in alignment with the iso 18385 standard. Each ladder is provided with a tube of 6x blueorange loading dye. Fullranger 100bp dna ladder 100bp 5000bp good range for small and large cloning applications. Tracking dye does not leave a shadow under uv light. Eleven fragments that range in size from 100bp to 1,000bp in 100bp increments plus a band at 1,500bp. Promega has developed a new formulation of this popular enzyme, gotaq dna polymerasea,b, as well as two new reaction buffers for use with this enzyme.
This study suggests that a combination of agnp treatment and dna pkcs inhibition may be a potential strategy to enhance the anticancer effect of agnp. Additional protocols for selected restriction enzymes promega scientists have tested a subset of restriction enzymes for compatibility with rapid digestion digesting dna in. For example, step ladder dna fragments could be in increments of 100bp from 100bp all the way up. To be the most responsive supplier of biological reagents and reagent systems used in research and applied technology applications worldwide. Dna ladders contain dna fragments of varying sizes. Promega dna ladder molecular weight markers, 100bp dna ladder. This study suggests that a combination of agnp treatment and dnapkcs inhibition may be a potential strategy to enhance the anticancer effect of agnp. The gotaq 1step rtqpcr system contains the proprietary fluorescent dnabinding dye, bryt green dye, that exhibits greater fluorescence enhancement upon binding to doublestranded dna dsdna, than does sybr green i. Dna and rna extraction, plasmid purification, reporter assays, pcr, qpcr, cloning enzymes, cell viability assays, apoptosis detection systems, luminometers, protein expression systems and more. Premixing ladder with 6x loading dye will shorten the expired date of it. Electrophoreses of dna ladder benchtop 100bp dna ladder. Feb 21, 2015 here we introduce a rapid and improved method of dna ladder apoptosis assay for evaluating apoptosis in mammalian cells.
The digested dna includes fragments ranging from 0. Thermo scientific generuler 1 kb dna ladder is designed for sizing and approximate quantification of wide range doublestranded dna on agarose gel. The dna ladder consists of proprietary plasmids which are digested to completion with appropriate restriction enzymes to yield 10 bands suitable for use as molecular weight standards for agarose gel electrophoresis. Pdf an update to dna ladder assay for apoptosis detection. For the convenience, the amounts of the 500 and 1,000 bp fragments are adjusted to show greater intensities100 bp dna ladder marker contains 11 double stranded dna fragments from 100bp to 1kb of 100bp increment and 1. Dna ladder is commonly used to determine the size of dna fragments by electrophoresis in routine. An update to dna ladder assay for apoptosis detection. While the technical manual indicates a dye dilution of 1. This marker is ideal for the size determination of digested dna. A highintensity 500bp band stands out at the lowest segment of the ladder 1kb. The key to accurate band analysis is to use the correct dna ladder for your particular application. Invitrogen dna ladders are available for a wide variety of size ranges, applications, and formats. Rq1 rna qualified rnase free dnase is a dnase i that degrades both doublestranded and singlestranded dna endonucleolytically, producing 3. Free sample available, use link below to request free sample highranger plus 100bp dna ladder 100bp 0bp analyzing dna over a wide range of sizes.
The authors further showed that dnapkcs may repair agnp induced dna damage by nhej and jnk1 pathways. The doublestranded dna is digested to completion with appropriate restriction enzymes, phenol extracted and equilibrated to 10 rnm trishci ph 8. Product components and storage conditions product size cat. All bands except the 800bp band are of equal intensity. Page 4 promega corporation2800 woods hollow road madison, wi 537115399 usa toll free in usa 8003569526 phone 6082744330 fax 6082772516. Then an update protocol of dna ladder assay was applied for detection of apoptosis. The quantifluor dsdna system is a highly accurate fluorescent method for quantifying dna. Promega 100bp dna ladder molecular weight marker 100 bp dna. In addition, dna pkcs may help recruit dna repair machinery to damaged telomeres. Promega 100bp dna ladder molecular weight marker 11 fragments from 100bp to 1,000bp in 100bp increments and includes an additional 1.
Promega 100bp dna ladder molecular weight marker life. Promega manufacturing and delivery systems continue to be fully operational during the covid19 outbreak. The ladder is composed of fourteen chromatographypurified individual dna fragments in base pairs. Thirteen bluntended fragments with sizes ranging from 250bp to 10,000bp. Wizard genomic dna purification kit protocol promega. The first step in the purification procedure lyses the cells and the. The 1,000bp and 3,000bp fragments have increased intensity relative to the other bands on ethidium bromidestained agarose gels for easy identification.
Summary of changes the following change was made to the 1218 revision of this document. The bio100bp dna ladder is supplied in 10mm triscl ph 8. Promega corporation is a worldwide leader in applying biochemistry and molecular biology to the development of innovative, highvalue products for the life sciences. Promega 100bp dna step ladder molecular weight marker for.
Straightforward procedure for laboratory production of dna ladder. Quickload 1 kb dna ladder is a premixed, readytoload molecular weight marker containing bromophenol blue as a tracking dye. Activation of cad by the caspase cascade leads to specific cleavage of the dna at the internucleosomal linker sites, generating fragments of 200 base. Premixing ladder with 6x loading dye will shorten the expired date of. The wizard genomic dna purification kit is designed for isolation of dna from white blood cells, tissue culture cells and animal tissue,plant tissue, yeast, grampositive and gramnegative bacteria. Dna ladders have defined sizes, and are not intended for use in quantitative analysis. Twelve dna fragments ranging from 25bp to 300bp in 25bp increments includes a visible 1800bp. The 1 kb and 3 kb bands contain more dna to provide internal orientation. Rq1 rnase free dnase is used in applications where maintaining the integrity of the rna is critical.
Quantifluor dsdna system for high concentration dna promega. For normal usage, premix the marker with 6x loading dye in ratio 5. Download a pdf containing pricing for our full product list. Two readytouse dna size markers for gel electrophoresis, the 100 bp ladder and 1 kb ladder represent the two most popular markers used for dna fragment size determination. Links to the entire contents of the promega product catalog are accessible from this page.
Promega 100bp dna step ladder molecular weight marker for electrophoresis forty bluntended dna fragments ranging from 100bp to 4,000bp in 100bp increments. The 1 kb dna ladder contains discrete dna fragments ranging in size from 300 bp to 10,000 bp. The ladders are not intended for use in quantitative analysis. Free pdf ebooks users guide, manuals, sheets about benchtop 1 kb dna ladder promega ready for download. Product components and storage conditions continued storage conditions. Often, dna molecular weight markers and ladders come in two formats. View more information about our dna stains technology. To create our dna ladders, each fragment is purified individually using proprietary chromatographybased technology, resulting in dna ladders of superior quality which exceeds industry standards. Learn how to interpret results from ystr dna typing using the ychromosome haplotype reference database yhrd, a free resource.
The ladder is composed of fourteen chromatographypurified. The approximate mass of dna in each of the bands is provided assuming a 0. The authors further showed that dna pkcs may repair agnp induced dna damage by nhej and jnk1 pathways. Quickload purple 1 kb dna ladder is stable for at least 6 months at 25c. Highranger 1kb dna ladder 300bp 0bp size determination of digested dna. This standard ensures minimal risk of human dna contamination for products used to collect, store and analyze. Promega notes number 83 2003 21 article subhead abstract gotaq dna polymerase, promegas new formulation of taq dna polymerase, is supplied with two buffersgreen gotaq reaction buffer and colorless gotaq reaction buffer. Dna ladder is commonly used to determine the size of dna fragments by. Our teams are in regular contact with suppliers and distributors worldwide and are taking all steps necessary to address both demands for diagnostic tools and reliable delivery of all products as quickly as possible. These ladders consist of equimolar mixtures of dna fragments for determining the mass of unknown dna samples on gels in the low and high molecular weight ranges. The concentration of 1kb dna ladder is 100 ugml 500ul five microlitres 500 ng of the 1 kb dna ladder are mixed with 1ul of blueorange 6x loading dye. Two internal features facilitate band identification. M marker benchtop 1 kb dna ladder promega corporation, madison, wi, usa. The ladder consists of bands that are generated from pcr and restriction enzyme digestion of double stranded dna.
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